Gelatin/chitin-degrading microorganisms are aerobic, meaning they prefer oxygen, so oxygen supply via an aerator is essential for cultivation.

Use the aerator until all nutrient solution is used.

It takes about a week to cultivate microorganisms using the product, and you must use them within 15 days after starting cultivation. This is when the microorganisms are most active. If too much time passes, other microorganisms may grow and contaminate the nutrient solution. (After use, the cultivation tank and heater need to be washed with water to remove any foreign substances.)

It is best to use the nutrient solution within 15 days of starting cultivation. Even if more than 15 days has passed and the microbial population and activity have decreased, the solution still contains many beneficial nutrients produced by microbes. Therefore, do not discard it, but apply it to the soil, making sure not to get any on the crop leaves.

Avoid direct sunlight and store at room temperature. (Storage temperature: 4°C to 35°C (39°F~95°F)

Generally recommend applying 2-3 times per month, with a maximum of 4 times. Soil drench is recommended at 10-15 day intervals, but can be more frequent depending on the crop’s growth condition. For foliar spray, diluting and using it in combination with pesticides or other eco-friendly agents can be more effective.

When cultivation occurs rapidly, a large amount of foam will be produced. Add about 1/3 cup of cooking oil in a regular paper cup. This will significantly reduce the amount of foam.

Cultivating Plus1000 in a 500L tank doesn’t result in a denser microbial cultivation. Using Plus1000 in a 500L tank yields a similar, slightly higher, number of microorganisms as cultivating Purne Plus.

Adding a large number of microorganisms at the initial stage of microbial cultivation does not necessarily double the multiplication rate compared to Purne Plus.

You can use fertilizer suited to the growth stage for secondary cultivation, but it must contain nitrogen, phosphate, and potash. You can reduce amounts but must include all three components.

Water temperatures between 25°C and 30°C (77°F to 86°F) are optimal for cultivating microorganisms. Temperatures above 40°C (104°F) can hinder microbial growth, so maintaining appropriate temperature is essential.

For a 500L cultivation product, up to 600L is possible. Cultivation beyond this capacity is difficult, but below this, cultivation is feasible. However, with smaller container, the microbial population increases proportionally to the container size. For example, a 300L container will culture 300L, and a 600L will culture 600L. Beyond that, cultivation becomes challenging due to insufficient microbial inoculum and nutrients.

The container is filled with water to about 3/4 full for cultivation because a large amount of foam may form during the process, risking overflow.

Rapid microbial proliferation causes a large amount of foam, while slow, gradual proliferation produces little foam. 

Regardless of foam production, by the end of cultivation period (approximately 6-7 days), microbial population reaches a similar level, indicating no issues with the cultivation process.